BioEnergy Science Center

Overcoming Recalcitrance

Biomass Formation and Modification

Enabling Technologies

Biomass Deconstruction and Conversion

Leading advancements in science and science-based innovation to remove economic barriers for cellulosic biofuels.

OUR NUMBERS

1

of 3 Centers

19

Licenses

58

Patent Applications

172

Invention Disclosures

Journal Articles

Citations

  • Industry Collaborations

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    Using a "commercialization council" of technology transfer and intellectual property (IP) management professionals from partner institutions, BESC evaluates the commercial potential of new inventions arising from BESC research and promotes and facilitates the licensing of BESC IP.

    Laboratory researcher
  • Education Outreach

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    BESC's education program, which is 75% self-sustaining, takes a novel approach. BESC has developed lesson plans to educate students about energy production and utilization. BESC uses a hub-and-spoke approach of working through regional science centers to maximize hands-on access and adaptation to local conditions.

    Researcher and student

Progress to Date

More Highlights


January 12, 2016

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Solubilization of washed mid-season switchgrass by various biocatalysts.

Optimizing Biocatalyst-Feedstock Combinations to Achieve High Solubilization with Minimal Pretreatment
This study carried out the most comprehensive controlled comparison to date of lignocellulosic solubilization by various biocatalysts, and report initial exploration of enhancing microbial solubilization of cellulosic biomass via mechanical disruption (cotreatment). Trends drawn from time-course and end-point data for six conversion systems and three substrates were: greater-than-expected differences in the effectiveness of various biocatalysts; lower yields and greater dependence on particle size for Populus as compared to green or senescent switchgrass; equal fractional solubilization of glucan and xylan with no biological solubilization of the non-carbohydrate fraction of biomass; and two-fold increase in solubilization via mechanical disruption with greater benefits on partially-fermented feedstock.


January 06, 2016

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Hemicellulose and Lignin

Review: How Plant Cell Wall NMR Provides Insights Into Biomass Chemistry and Structures
The structure of the plant cell wall and recalcitrance are intimately tied together such that fundamental breakthroughs in recalcitrance have driven the science of biomass characterization. Work from BESC and other energy centers has dramatically enhanced the resolution and productivity of Nuclear Magnetic Resonance (NMR) analysis of biomass polymers including lignin, cellulose and hemicellulose. Whole cell NMR analysis, as reviewed in this article, provides a relatively facile means of characterizing changes in lignin and hemicellulose that contribute to reduced recalcitrance in pretreated biomass and/or transgenic or natural variant plant species.


December 31, 2015

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Comparison of cell wall (1) and growth (2) traits between T2-generation transgenic (+) and non-transgenic null-segregant (-) hybrid families.

Successful Transfer of Transgenic Switchgrass Low-Recalcitrance Traits into High-Yielding Field Variety
This study evaluated the feasibility of transferring the low-recalcitrance traits associated with a transgenic switchgrass line into high-yielding field varieties in an attempt to improve growth-related traits and to preserve the transgenic benefit. Our results provide insights into the possible improvement of switchgrass productivity via biotechnology paired with plant breeding.


December 26, 2015

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Root enhancement in winter wheat due to S. vermifera colonization in vitro.

Review: Potential Impact of S. vermifera, A Unique Root Symbiont, On Agronomics of Biofeedstocks
Research performed with orchid mycorrhizal fungus Sebacina vermifera clearly indicates significant growth-promoting abilities in a remarkably broad spectrum of plants, including switchgrass, that rivals or even surpasses that of arbuscular mycorrhizae. Sebacinoid fungi should be considered as a previously hidden, but amenable and effective microbial tool for enhancing plant productivity and stress tolerance which has been validated in switchgrass, an important biofeedstock for biofuels.


December 14, 2015

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System-wide Redox Stress Response: red indicates decreased transcription, (#) is the number of genes in the pathway decreased.

Towards Understanding Gene Systems Mediating Redox Homeostasis and Energy Metabolism in C. thermocellum
Current moderate ethanol yields and productivities as well as growth inhibition impede industrial deployment of this bacterium for commodity fuel production. Redox imbalances may contribute to incomplete substrate utilization, limit bioproductivity, and direct fermentation products to undesirable overflow metabolites. The redox systems identified are active and fungible in C. thermocellum upon introduction of a redox stress, and considered a link between redox stress and the occurrence of ethanol yield-limiting, unwanted metabolic processes. This expression study informs ongoing metabolic engineering efforts to increase bioproductivity in C. thermocellum.


December 10, 2015

Commercial Licensing of a BESC Global Genetic Regulator of Aromatic Biosynthesis
BESC has been studying SNPs and key compositional and conversion phenotypes in the natural variation population in Populus using GWAS (genome-wide association studies). A study of rare SNPs identified an unusual paralog in Populus which had taken on new regulator functions. Patent applications have been filed. Follow-on work is testing the utility in other plant species. The technology is being licensed to two companies who plan to commercialize the technology: GreenWood Resources and Forest Genetics International. Increasing biofuel yield or forage digestibility can lead to reductions in land use for these crops and thus contributing to long term sustainability.

More Publications

Publication Date Citation
DOI

BioEnergy Science Center one of three DOE Bioenergy Research Centers established by the U.S. Department of Energy.