BioEnergy Science Center

Overcoming Recalcitrance

Biomass Formation and Modification

Enabling Technologies

Biomass Deconstruction and Conversion

Leading advancements in science and science-based innovation to remove economic barriers for cellulosic biofuels.

OUR NUMBERS

1

of 3 Centers

19

Licenses

58

Patent Applications

172

Invention Disclosures

Journal Articles

Citations

  • Industry Collaborations

    >

    Using a "commercialization council" of technology transfer and intellectual property (IP) management professionals from partner institutions, BESC evaluates the commercial potential of new inventions arising from BESC research and promotes and facilitates the licensing of BESC IP.

    Laboratory researcher
  • Education Outreach

    >

    BESC's education program, which is 75% self-sustaining, takes a novel approach. BESC has developed lesson plans to educate students about energy production and utilization. BESC uses a hub-and-spoke approach of working through regional science centers to maximize hands-on access and adaptation to local conditions.

    Researcher and student

Progress to Date

More Highlights


August 25, 2016

Heterologous Expression of Xylanases from A. cellulolyticus Enhances Hemicellulolytic Activity of C. bescii
To test whether the addition of A. cellulolyticus xylanases might enhance the hemicellulolytic activity of C. bescii and growth on xylan substrates, the xylanases were expressed in C. bescii using new expression vectors. Expression of the xylanases in C. bescii results in an increase in the activity of the exoproteome on xylan and a dramatic increase in its ability to grow on xylan substrates.


August 10, 2016

Microbial Upgrading of Lignin-Derived Carboxylates to Branched-Chain Esters for Drop-in Biofuels
An innovative biological conversion route was developed for conversion of lignocellulosic biomass or organic wastes into biofuels and high-value chemicals.


August 08, 2016

image
Cellulose in THF-Water

HPC Simulation of Co-Solvent Pretreatment of Biomass Confirmed by Experiment
Previous BESC work has shown that reaction of biomass in aqueous tetrahydrofuran (THF) in CELF (Co-solvent Enhanced Lignocellulosic Fractionation) pretreatment is highly effective at delignification and deconstruction of biomass for biofuels and bioproducts. Molecular dynamics simulations were performed of whole cellulose fibers and single cellulose chains in a THF-water co-solvent at various temperatures on the ORNL TITAN supercomputer. The simulations were complemented by THF-water experiments on cellulose, measuring its solubilization and imaging the product residue.


July 27, 2016

image
One-hour hydrolysis rate versus cumulative cellulose conversion for uninterrupted and restarted hydr (...more)

Cellulose DP And Accessibility Contribute To Cellulase Enzyme Action On Biomass Substrate
The enzymatic hydrolysis of lignocellulosic biomass proceeds initially at a fast rate followed by a rapid decrease in the conversion rates. Degree of polymerization (DP) analysis during hydrolysis suggested a synergistic action of endo- and exo-glucanases that contribute to the occurrence of a peeling off mechanism. Cellulose accessibility analysis showed that limited accessible surface area of cellulose is probably not a major limiting factor that causes the decline of hydrolysis rate in its late stage. Restart hydrolysis experiment suggested that enzyme related factors such as enzyme inactivation or steric hindrance of enzymes should be responsible to the reduction in hydrolysis rate given the large size of cellulase enzymes.


June 21, 2016

image
Dr. Liao received his Bachelor degree at the National Taiwan University and his Doctorate at the University of Wisconsin-Madison

James Liao Appointed To Serve As Academia Sinica President
Dr. James Liao has been named as the 11th president of Academia Sinica — the top academic research institution in Taiwan. Academia Sinica supports research activities in a wide variety of disciplines, ranging from mathematical and physical sciences, to life sciences and to humanities and social sciences. Dr. Liao serves as the BESC Activity Lead for Butanol Production by CBP.


June 20, 2016

Improved Thermophilic Anaerobe Achieves High Biofuel Titers From Wood And Cellulose
This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates. However,challenges were encountered with existing fungal cellulases and inhibitors from pretreatment. The high titers and yields observed support the feasibility of using engineered thermophiles for industrial ethanol production if challenges associated with pretreatment inhibitors can be avoided.


June 20, 2016

image
Relation between viscosity and shear rate of poplar lignin at 170 °C

Syringyl/Guaiacyl (S/G) Ratio In Lignin May Influence Quality Of Carbon Fiber Produced From Lignins
Current lignin-based carbon fiber using low-cost lignin precursors does not possess the mechanical properties required for many structural applications. Lignins with various syringyl/guaiacyl (S/G) ratios were isolated from Populus genotypes and subjected to rheological treatment at various temperatures and cooling conditions. Organosolv lignins extracted from genotypes with lower S/G ratios exhibited higher viscosity values and molecular weights with larger amounts of condensed structures following rheology treatment at relatively lower temperature; higher viscosity and molecular weight is known to provide for higher quality carbon fiber.


June 20, 2016

image
Isolation and characterization of M. truncatula mutant seeds lacking physical dormancy.

Discovery Of A Novel Molecular Mechanism Controlling Seed Physical Dormancy
Physical seed dormancy exists widely in higher plants, but its molecular mechanism has remained largely elusive. By screening a large number of Tnt1 retrotransposon-tagged Medicago truncatula lines, we identified non-dormant seed mutants from this model legume species. This study elucidated a novel molecular mechanism of physical dormancy and revealed a new role of class II KNOX genes.


June 07, 2016

Simultaneous Achievement Of High Ethanol Yield And Titer In Clostridium thermocellum
Adaptive evolution successfully improved the ethanol yield and the titer from a rationally engineered CBP strain. This evolved strain has the highest ethanol yield and titer reported to date for C. thermocellum, and is an important step in the development of this microbe for industrial applications.


June 01, 2016

Workshop Summary: Lessons From Industry In Commercializing Biotechnology
This article summarizes lessons learned drawn from the "Technology Challenges and Opportunities in Commercializing Industrial Biotechnology Workshop," September 28-29, 2015, San Diego.


May 09, 2016

A Bifunctional Ammonia-Lyase Is Critical In Grass Cell Wall Biosynthesis Of Lignin
Using phylogenetic, transcriptomic and in vitro biochemical analyses, a single homotetrameric cytosolic bifunctional ammonia-lyase (PTAL) was identified among eight BdPAL enzymes in the model grass species Brachypodium distachyon. 13C stable isotopic labelling experiments along with BdPTAL1-down-regulation in transgenic plants showed that the TAL activity of BdPTAL1 can provide nearly half of the total lignin deposited in grasses, with a significant preference for S-lignin and wall-bound coumarate biosynthesis.


April 29, 2016

image
Ball-milled whole cell wall poplar samples in different NMR solvents.

Improved Whole Cell Wall NMR Measures Carbohydrate And Lignin Structures Without Derivatization
A new NMR solvent system composed of dimethylsulfoxide (DMSO-d6) and hexamethylphosphoramide (HMPA-d18) was developed to understand plant cell wall structures with minimal deconstruction and modification of biomass. The proposed bi-solvent system does not require derivatization or chemical modification, thus it facilitates easier sample preparation and less signal overlapping of correlation peaks compared to the traditional methods.


April 28, 2016

image
BRC Directors

Intellectual Property Impact of the BRCs
The Bioenergy Science Centers (BRCs) have generated over 500 invention disclosures, 328 patent applications (of which 45 have been awarded) which have led to 133 licenses or options with industry.


April 28, 2016

image
Reduction of lignin content.

Identification And Overexpression Of Knotted1-Like Transcription Factor For Lignocellulosic Feedstock Improvement
Using transgenic approach, a switchgrass Knotted1 transcription factor, PvKN1 was overexpressed in switchgrass and transgenic plants were characterized for recalcitrance and growth-related traits. PvKN1 overexpression resulted in reduced lignin deposition/biomass recalcitrance and improved sugar release efficiency without affecting various growth parameters such as tiller height, tiller number, fresh and dry biomass.


April 26, 2016

image
QTL for lignin content and glucose release

New Genetic Markers Identified For Switchgrass Cell Wall Composition And Conversion
A full-sib switchgrass population was developed by crossing a lowland genotype (AP13) to an upland genotype (VS16). QTL associated with the traits of interest were identified. Significant variation for glucose, xylose and total sugar release, and lignin content (19-27%) in the population; a unit increase in lignin content reduced total sugar release by an average of 10 mg g-1; and QTL analysis detected nine genomic regions underlying sugar release and 14 for lignin content.


April 26, 2016

Structural Diversity Of Xylans Measured In Cell Walls Of Monocots
This study provides a new perspective on the diversity, function and evolution of monocot cell walls, enabling the manipulation of xylan structure and development of new technologies for the conversion of biomass from this important family of plants to yield commercially valuable and sustainable products.


April 26, 2016

image
adhE expression and ethanol production with old vs. new plasmids

Development Of Improved Expression Plasmid In Clostridium thermocellum
Clostridium thermocellum is a promising candidate for ethanol production from cellulose biomass yet requires metabolic engineering to further improve ethanol yield. An expression plasmid was redesigned to improve transformation efficiency and structural stability. The new expression plasmid had both significantly higher transformation efficiency than its predecessor (~2x more), and was also able to more robustly express genes of interest.


April 25, 2016

BRCs Well Represented at Symposium on Biotechnology for Fuels and Chemicals
The Symposium on Biotechnology for Fuels and Chemicals (SBFC) was held April 25-28, 2016, in Baltimore, Maryland. As part of the overall symposium the combined Bioenergy Research Centers (BRCs) presented thirty-seven talks and twenty posters representing 25% of the talks and 10% of the posters.


April 18, 2016

image
Photo of the field in June 2013.

Lignification And Recalcitrance Affected By Age And Season In Field-Grown COMT-Downregulated Switchgrass
Previous field and greenhouse studies have shown that COMT-downregulation in switchgrass decreases lignin content and reduces the S/G ratio, thereby lowering recalcitrance and improving ethanol yields. Trends in lignification and recalcitrance differ between growing seasons. Third-year results showed an increase in lignification and decrease in sugar release across the season. S/G ratios increased over time in non-transgenic control lines, but were relatively stable in COMT-downregulated lines. During early (elongation) growth stages, cell wall chemistry was similar between COMT-downregulated and control lines. After reaching the reproductive growth stage in July, COMT-downregulated lines maintained reduced lignin content, decreased S/G ratios, and improved sugar release through December. No differences in rust susceptibility were observed.


April 08, 2016

image
Dr. Willington Muchero, lead ORNL inventor

GreenWood Resources, Inc. Licenses BESC Invention For Lignin Regulator
Studies on the natural variation in lignin content in poplar trees identified a gene with major effects on lignin biosynthesis, the key polymer hindering efficient biomass digestibility. GreenWood Resources, Inc. has nonexclusively licensed this jointly-owned BESC invention and will continue to partner with ORNL as they complete field studies.


April 01, 2016

BioEnergy Science Center (BESC) Website Receives A "Distinguished Technical Communication" Award
The Washington-Baltimore Society for Technical Communication (STC) recognized the BioEnergy Science Center (BESC) website with the "Distinguished Technical Communication" award in April 2016.


March 30, 2016

image
Overall schemes of fuels from sunlight and atmospheric carbon.

Review: Using Microorganisms For The Production Of Next-Generation Biofuels
A review on how microorganisms can be exploited for the production of next-generation biofuels: a) based on the ability of bacteria and fungi to use lignocellulose; b) via direct CO2 conversion using microalgae; c) lithoautotrophs driven by solar electricity; or d) relying on the capacity of microorganisms to use methane generated from landfill.


February 05, 2016

image
TEM micrographs of Avicel particles after incubation with Clostridium thermocellum

Discovery Of New Enzyme System Sheds Further Light On High Cellulolytic Performance Of C. thermocellum
C. thermocellum utilizes not only all the cellulase degradation mechanisms known today (cellulosomes and free enzymes), but also a new category of scaffolded enzymes not attached to the cell. This discovery explains C. thermocellum's superior performance on biomass. These scaffoldins were found to be essential for the well known digestion mechanism, i.e., the defibrillation of plant cell walls.


February 04, 2016

image
CBP conversion of untreated Populus with high S/G ratio of lignin yielded significantly better hydrolysis and fermentation products.

Contribution Of Lignin Composition And Structure To Improvements In Bioprocessing Of Populus Using CBP
Populus variants with low and high syringyl to guaiacyl (S/G) ratios were hydrolyzed and fermented with Clostridium thermocellum under CBP conditions, and compared to conventional fungal enzymes and yeast. The composition and structure of cellulose and lignin in the biomass was correlated with the bioconversion results. Samples with a S/G ratio of 2:1 were converted more rapidly and to a greater extent than biomass with a 1:2 S/G ratio. The CBP microbe hydrolyzed biomass two to three times more readily than commercial fungal enzymes in either scenario. Lignin polymer size (lignin Mw) was found to be lower for the low S/G biomass.

More Publications

Publication Date Citation
DOI

BioEnergy Science Center one of three DOE Bioenergy Research Centers established by the U.S. Department of Energy.