Enabling Technologies

Research Overview | Research Publications | Intellectual Property

Simulating Cell Wall Degradation. BESC researchers are using computational modeling to gain a molecular-level understanding of the plant cell wall and its major components, including cellulose (green) and lignin (brown). Here, the enzyme cellulase (orange) catalyzes cell wall breakdown. [Illustration courtesy Thomas Splettstoesser, www.scistyle.com, for Oak Ridge National Laboratory]

The BioEnergy Science Center (BESC) has developed a suite of new enabling technologies. These include characterization and computational modeling efforts, which focused on developing and applying chemical, immunological, physical, and imaging methods to characterize biomass. The resulting information is then used to identify relationships between biomass structure and recalcitrance.

Other new enabling technologies support and enhance feedstock and consolidated bioprocessing (CBP) research. These technologies include high-throughput (HTP) assays for determining the recalcitrance properties of tens of thousands of feedstock samples. The information obtained from HTP pipelines for pretreatment, digestibility, and chemistry has enabled plant scientists to discover improved plant lines and single-nucleotide polymorphisms (SNPs) that link genotypic variation to cell wall chemistry in natural populations of poplar and switchgrass.

Tests that integrate enhanced-biomass grasses with cellulolytic microorganisms are showing greater effectiveness in conversion and biofuel production, supporting a BESC hypothesis that minimal to no pretreatment may be possible for some conversion regimes.

Modeling and simulation tools developed by BESC researchers are being used to analyze various aspects of recalcitrance. Flux-balance analyses have provided insight into lignin synthesis, and several tools are publicly available within the BESC KnowledgeBase to analyze, for example, carbohydrate-active enzymes (CAZymes) and regulons. These enabling technologies not only have advanced the science of biomass production more quickly, but also can be applied to numerous other areas of biological research.


Enabling Technologies Research Results

Publications

  Publication Date Citation
DOI
  11/25/2014 Bali, G., Meng, X., Deneff, J.I., Sun, Q., Ragauskas, A.J., "The effect of alkaline pretreatment methods on cellulose structure and accessibility", ChemSusChem Communications, 2014
10.1002/cssc.201402752
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  11/01/2014 Carmona, C., Langan, P., Smith, Smith, J.C., Petridis, L., Why genetic modification of lignin leads to low-recalcitrance biomass, Physical Chemistry Chemical Physics, 1-3, 2014
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  10/14/2014 Sun, Q., Foston, M., Meng, X., Pingali, V., O'Neill, Li, H., Wyman, C., Langan, P., Ragauskas, A., Kumar, R., The Effect of Lignin Content on Changes that Occur in Poplar Cellulose Ultrastructure during Dilute Acid Pretreatment, Biotechnology for Biofuels, 7:150, 2014.
10.1186/s13068-014-0150-6
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  10/14/2014 Chai, J., Kora, G., Ahn, T-H., Hyatt, D., Pan, C., Functional phylogenomics analysis of bacteria and archaea using consistent genome annotation with UniFam, BMC Evolutionary Biology, 14:207, 2014.
10.1186/s12862-014-0207-y
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  10/11/2014 Singh, S., Cheng, G., Sathitsuksanoh, N., Wu, D., Varanasi, P., George, A., Balan, V., Gao, X., Kumar, R., Dale, B., Wyman, C., Comparision of different biomass pretreatment techniques and their impact on chemistry, structure, and recalcitrance, Frontiers in Energy Research, 2014
  09/18/2014 Qin, M., Chen, X., Chao, L., XiZeng, M., Yuan, Z.H., Fei, J.L., ChunGuo, W.U., Ying, X.U., Understanding the commonalities and differences in genomic organizations across closely related bacteria from an energy perspective, Science China Life Science, 2014
10.1007/s11427-014-4734-y
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  09/11/2014 Yang, S., Franden, M.A., Brown, S.D., Chou, Y.C., Pienkos, P.T., Zhang, M., Insights into acetate toxicity in Zymomonas mobilis 8b using different substrates, Biotechnology for Biofuels, 2014
10.1186/s13068-014-0140-8
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  09/08/2014 Zaharov, V.V., Farahi, R.H., Snyder, P.J., Davison, B.H., Passian, A., Karhunen-Lo`eve treatment to remove noise and facilitate data analysis in sensing, spectroscopy and other applications, Analyst, 139:5927 2014
10.1039/C4AN01300J
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  09/02/2014 Mostofian, B., Cheng, X., Smith, J.C., Replica-exhange molecular dynamics simulations of cellulose solvated in water and in the ionic liquid 1-butyl-3-methylimidazolium choloride, The Journal of Physical Chemistry B, 118, 11037-11049, 2014.
10.1021/jp502889c
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  08/16/2014 Linville, J.L., Rodriguez Jr., M., Brown, S.D., Mielenz, J.R., Cox, C.D., Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain, BMC Microbiology, 14:215, 2014.
10.1186/s12866-014-0215-5
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  08/01/2014 Pyne, M.E., Utturkar, S., Brown, S.D., Moo-Young, M., Chung, D.A., Cho, C.P., Improved draft genome sequence of Clostridium pasteurianum strain ATCC 6013 (DSM 525) using a hybrid next-generation sequencing approach, Genome Announcements, 2(4), 2014.
10.1128/genomeA.00790-14
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  07/28/2014 Akinosho, H., Yee, K., Close, D., Ragauskas, A.J., The emergence of clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications, Frontiers in Chemistry, 2014
10.3389/fchem.2014.00066
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  07/01/2014 Lupoi, J.S., Singh, S., Davis, M., Lee, D.J., Shepherd, M., Simmons, B.A., Henry, R. J., High-throughput prediction of eucalypt lignin syringyl/guaiacyl content using multivariate analysis: a comparison between mid-infrared, near-infrared, and Raman spectroscopies for model development, Biotechnology for Biofuels, 7:93, 2014.
10.1186/1754-6834-7-93
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  07/01/2014 Woo, H.L., Utturkar, S., Klingeman, D., Simmons, B.A., DeAngelis, K.M., Brown, S.D., Hazen, T.C., Draft genome sequence of the lignin-degrading Burkholderia sp. Strain LIG30, isolated from wet tropical forest soil, 2, 2014
10.1128/genomeA.00637-14
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  07/01/2014 Li, Y., Xu, T., Tschaplinski, T.J., Engle, N.L., Yang, Y., Graham, D.E., He, Z., Zhou, J., Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation, Biotechnology for Biofuels, 7:25, 2014.
10.1186/1754-6834-7-25
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  06/03/2014 Zhou, C., Mao, F., Yin, Y., Huang, J., Gogarten, J.P., Xu, Y., AST: An automated sequence-sampling method for improving the taxonomic diversity of gene phylogenetic trees, 9(6), 2014.
10.1371/journal.pone.0098844
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  06/03/2014 Zhou, C., Ma, Q., Mao, X., Yin, Y., Xu, Y., New Insights into Clostridia through Comparative 1 Analyses of Their 40 Genomes, Bioenergy Research, 2014
10.1007/s12155-014-9486-9
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  05/22/2014 Yee, K.L., Rodriguez Jr., M., Thompson, O.A., Fu, C., Wang, Z.Y., Davison, B.H., Mielenz, J.R., Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain, Biotechnology for Biofuels, 7:75, 2014.
10.1186/1754-6834-7-75
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  05/22/2014 Yang, S., Pan, C., Hurst, G.B., Dice, L., Davison, B.H., Brown, S.D., Elucidation of zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics, Frontiers, 5:246 2014
10.3389/fmicb.2014.00246
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  05/15/2014 Ragauskas, A.J., Beckham, G.T., Biddy, M.J., Chandra, R., Chen, F., Davis, M.F., Davison, B.H., Dixon, R.A., Gilna, P., Keller, M., Langan, P., Naskar, A.K., Saddler, J.N., Tschaplinski, T.J., Tuskan, G.A., Wyman, C.E., Lignin Valorization: improving lignin processing in the biorefinery, Science, 344, 2014.
10.1126/science.1246843
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  05/14/2014 Sun, Q., Foston, M., Sawada, D., Pingali, S.V., O'Neill, H.M., Li, H., Wyman, C.E., Langan, P., Pu, Y., Ragauskas, A.J., Comparison of changes in cellulose ultrastructure during different pretreatments of poplar, Cellulose, 21 (2419-2431) 2014
10.1007/s10570-014-0303-6
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  05/13/2014 Gao, X., Kumar, R., Singh, S., Simmons, B.A., Balan, V., Dale, B.E., Wyman, C.E., Comparison of Enzymatic reactivity of corn stover solids prepared by dilute acid, AFEXtm, and ionic liquid pretreatments, Biotechnology for Biofuels, 7:71, 2014.
10.1186/1754-6834-7-71
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  04/21/2014 Ma, Q., Zhang, H., Mao, X., Zhou, C., Liu, B., Chen, X., Xu, Y., DMINDA: An integrated web server for DNA motif identification and analyses, Nucleic Acids Research, 42:W1-W12 2014
10.1093/nar/gku315
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  04/14/2014 Tolbert, A., Akinosho, H., Khunsupat, R., Naskas, A.K., Ragauskas, A.J., Characterization and analysis of the molecular weight of lignin for biorefining studies, Biofuels, Bioproducts and Biorefining, 2014
10.1002/bbb.1500
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  04/13/2014 Uppugundia, N., da Costa Sousa, L., Chundawat, S., Yu, X., Simmons, B., Singh, S., Gao, X., Kumar, R., Wyman, C.E., Dale, B.E., Balan V., A comparative study of ethanol production using dilute acid, ionic liquid and AFEXTM pretreated corn stover, Biotechnology for Biofuels, 7:72, 2014.
10.1186/1754-6834-7-72
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  04/04/2014 Li, H., Pattathil, S., Foston, M.B., Ding, S.Y., Kumar, R., Gao, X., Mittal, A., Yarbrough, J.M., Himmel, M.E., Ragauskas, A.J., Hahn, M.G., Wyman, C.E., Agave proves to be a low recalcitrant lignocellulosic feedstock for biofuels production on semi-arid lands, Biotechnology for Biofuels, 7:50, 2014
10.1186/1754-6834-7-50
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  04/03/2014 Liu, T., Williams, D.L., Pattathil, S., Hahn, M.G., Li, M., Hodge, D.B., Coupling alkaline pre-extraction with alkaline-oxidative post-treatment of corn stover to enhance enzymatic hydrolysis and fermentability, Biotechnology for Biofuels, 7:48 2014
10.1186/1754-6834-7-48
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  04/01/2014 Kumagai, A., Kawamura, S., Lee, S.H., Endo, T., Rodriguez Jr., M., Mielenz, J. R., Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling, Bioresource Technology, 162:89-95, 2014.
10.1016/j.biortech.2014.03.110
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  03/26/2014 Li, M., Pattathil, S., Hahn, M.G., Hodge, D., Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling, RSC Advances, 4:33 (17282-17292) 2014
10.1039/C4RA00824C
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  03/21/2014 Brown, S.D., Nagaraju, S., Utturkar, S., De Tissera, S., Segovia, S., Mitchell, W., Land, M.L., Dassanayake, A., Kopke, M., Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPER systems in industrial relevant Clostridia, BioTechnology for Biofuels, 7:40, 2014.
10.1186/1754-6834-7-40
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  03/21/2014 Lindedam, J., Bruun, S., Jorgensen, H., Decker, S.R., Turner, G.B., DeMartini, J.D., Wyman, C.E., Felby, C., Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production, Biomass and Bioenergy, 66 (261-267) 2014
10.1016/j.biombioe.2014.03.006
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  03/01/2014 Robinson, W., Farming for Fuels, Dimensions, 16:2 (46-48) 2014
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  03/01/2014 Chou, W.C., Ma, Q., Yang, S., Cao, Klingeman, D.M., Brown, S.D., Xu, Y., Analysis of strand-specific RNA-seq data using machine learning reveals the structures of transcription units in Clostridium thermocellum, Nucleic Acids Research, 2014
  03/01/2014 Edwards, M.C., Williams, T., Pattathil, S., Hahn, M.G., Peterson-Doran, J., Replacing a suite of commercial pectinases with a single enzyme, pectate lyase B, in Saccharomyces cerevisiae fermentations of cull peaches, Journal of Microbiology and Biotechnology, 41:679-686, 2014.
10.1007/s10295-013-1394-z
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  02/12/2014 Kumar, R., Wyman, C.E., Strong cellulase inhibition by mannan polysaccharides in cellulose conversion to sugars, Biotechnology and bioengineering, 111:7 (1341-1353) 2014
10.1002/bit.25218
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  02/12/2014 Karpinets, T.V., Park, B.H., Syed, M.H., Klotz, M.G., Uberbacher, E.C., Metabolic Environments and Genomic Features Associated with Pathogenic and Mutualistic Interactions Between Bacteria and Plants, Molecular Plant-Microbe Interactions, 27:7 (664-667) 2014
10.1094/MPMI-12-13-0368-R
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  02/01/2014 Meng, X., Ragauskas, A.J., Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates, Current Opinion in Biotechnology, 27, 150-158, 2014.
10.1016/j.copbio.2014.01.014
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  02/01/2014 Shields-Menard, S.A., Brown, S.D., Klingeman, D.M., Indest, K., Hancock, D., Wewalwela, J.J., French, W.T., Donaldson, J.R., Draft genome sequence of Rhodococcus rhodochrous Strain ATCC 21198, Genome Announcements, 2:1 2014
10.1128/genomeA.00054-14.
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  01/30/2014 Penning, B.W., Sykes, R.W., Babcock, N.C., Dugard, C.K., Klimek, J.F., Gamblin, D., Davis, M., Filley, T.R., Mosier, N.S., Weil, C.F., McCann, M.C., Carpita, N.C., Validation of PyMBMS as a high-throughput screen for lignin abundance in lignocellulosic biomass of grasses, Bioenergy Research, 7:3 (899-908) 2014
10.1007/s12155-014-9410-3
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  01/20/2014 Samuel, R., Pu, Y., Jiang, N., Fu, C., Wang, Z.Y., Ragauskas, A., Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass, Frontiers in Energy Research, 14;1, 2014.
10.3389/fenrg.2013.00014
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  01/07/2014 Biswal, A.K., Soeno, K., Gandla, M.L., Immerzeel, P., Pattathil, S., Lucenius, J., Serimaa, R., Hahn, M.G., Moritz, T. Jonsson, L.J., Isralelsson-Nordstrom, M., Mellerowica, E.J., Aspen pectate lyase PtxtPL1-27 mobilizes matrix plysaccharides from woody tissues and improves saccharification yield, Biotechnology for Biofuels, 7:11, 2014.
10.1186/1754-6834-7-11
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  12/30/2013 Sangha, A.K., Davison, B.H., Standaert, R.F., Davis, M.F., Smith, J.C., Parks, J.M., Chemical factors that control lignin polymerization, The Journal of Physical Chemistry B, 118:1 (164-170) 2014
10.1021/jp411998t
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  12/17/2013 Gao, X., Kumar, R., Wyman, C.E., Fast hemicellulose quantification via a simple one-step acid hydrolysis, Biotechnology and Bioengineering, 111:6 (1088-10963) 2013
10.1002/bit.25174
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  12/16/2013 Hu, F., Ragauskas, A., Suppression of pseudo-lignin formation under dilute acid pretreatment conditions, RCS Advances, 4, 4317-4323, 2014.
10.1039/C3RA42841A
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  12/12/2013 Fineran, P.C., Cans Igleslas, M.C., Ramsay, J.P., Wilf, N.M., Cossyleon, D., McNeil, M.B., Williamson, N.R., Monson, R.E., Becher, S.A., Stanton, J.L., Brugger, K., Brown, S.D., Salmond, G.P.C., Draft Genome Sequence of Serratia sp. Strain ATCC 39006, a Model Bacterium for Analysis of the Biosynthesis and Regulation of Prodigiosin, a Carbapenem, and Gas Vesicles, 1(6), 2013.
10.1128/genomeA.01039-13.
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  12/12/2013 Ding, S.Y., Zhao, S., Zeng, Y., Size, shape, and arrangement of native cellulose fibrils in maize cell walls, Cellulose, 21:2 (863-871) 2013
10.1007/s10570-013-0147-5
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  12/02/2013 Wilson, C.M., Rodriguez Jr., M., Johnson, C.M., Martin, S.L., Chu, T.M., Wolfinger, R.D., Hauser, L.J., Land, M.L., Klingeman, D.M., Syed, M.H., Ragauskas, A.J., Tschaplinski, T.J., Mielenz, J.R., Brown, S.D., Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass, Biotechnology for Biofuels, 6:179, 2013.
10.1186/1754-6834-6-179
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  11/20/2013 Iwata, M., Shiraishi, F., Voit, E.O., Coarse but efficient identification of metabolic pathway systems, International Journal of Systems Biology, 4:1 (57-72) 2013
0975-2900 & E-ISSN: 0975-9204,
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  11/18/2013 Liu, Y.S., Ding, S.Y., Advances in the measurement and characterization of biomass structure and processing, Biological Concerstion of Biomass for Fuels and Chemicals: Explorations from Natural Utilization Systems, 10:3 (26-36) 2013
10.1039/9781849734738-00026
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  11/07/2013 Mao, X., Ma, Q., Zhou, C., Chen, X., Zhang, H., Yang, J., Mao, F., Lai, W., Xu, Y., DOOR 2.0: presenging operons and their functions through dynamic and integrated views, Nucleic Acids Research, 1-6, 2013.
10.1093/nar/gkt1048
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  10/21/2013 Linville, J.L., Rodriguez Jr., M., Land, M., Syed, M., Engle, N.L., Tschaplinski, T.J., Mielenz, J.R., Cox, C.D., Industrial robustness: understanding the mechanism of tolerance for the populus hydrolysate-tolerant mutant strain of clostridium thermocellum, Plos One, 8:10 e78829 2013
10.1371/journal.pone.0078829
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  10/12/2013 Li, H., Pu, Y., Kumar, R., Ragauskas, A.J., Wyman, C.E., Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms, 111:3, 2013.
10.1002/bit.25108
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  10/01/2013 Ratnaparkhe, S., Venkatachalam, S., Hah, M.G., Pattathil, S., Analyses using cell wall glycan-directed monoclonal antibodies reveal xylan-degration by two microbial glycosyl hydrolases in cell walls from poplar and switchgrass biomass, Journal of Bioremediation & Biodegradation, S4:004, 2013.
10.4172/2155-6199.S4-004
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  09/12/2013 Wilson, C.M., Yang, S., Rodriguez Jr., M., Ma. Q., Johnson, C.M., Dice, L., Xu, Y., Brown, S.D., Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress, Biotechnology for Biofuels, 6:131, 2013
doi:10.1186/1754-6834-6-131
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  09/04/2013 GhattyVenkataKrishna, P.K., Mostofian, B., Dynamics of water in the amphiphilic pore of amyloid b fibrils, Chemical Physics Letters, 582, 1-5, 2013.
10.1016/j.cplett.2013.07.026
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  09/01/2013 Voit, E.O., Characterizability of metabolic pathway systems from time series data, Mathematical Biosciences, 246:2 (315-325) 2013
10.1016/j.mbs.2013.01.008
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  09/01/2013 Voit, E.O., Biochemical Systems Theory: A Review, ISRN Biomathematics, 53, (897658) 2013
10.1155/2013/897658
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  08/20/2013 Linville, J.L., Rodiguez Jr., M., Mielenz, J. R., Cox, C.D., Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum, Bioresource Technology, 147, pp 605-613, 2013.
10.1016/j.biortech.2013.08.086
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  08/01/2013 Mostofian, B., Smith, J.C., Cheng, X., Simulation of a cellulose fiber in ionic liquid suggests a synergistic approach to dissolution, 21(2):938-997, 2014.
10.1007/s10570-013-0018-0
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  08/01/2013 Lian, P., Guo, H.B., Smith, J.C., Wei, D.Q., Gui, H, Catalytic mechanism and origin of high activity of cellulase TmCel12A at high temperature: a quantum mechanical/molecular mechanical study, Cellulose, 2013
10.1007/s10570-013-0011-7
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  08/01/2013 Trajano, H.L., Engle, N.L., Foston, M., Ragauskas, A.J., Tschaplinski, T.J., Wyman, C.E., The fate of lignin during hydrothermal pretreatment, Biotechnology for Biofuels, 6:110; 2013.
10.1186/1754-6834-6-110
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  07/16/2013 Yang, S., Pan, C., Tschaplinski, T.J., Hurst, G.B., Engle, N.L., Zhou, W., Dam, P., Xu, Y., Rodriguez Jr. M., Dice, L., Johnson, C.M., Davison, B.H., Brown, S.D., Systems biology analysis of zymomonas mobilis ZM4 ethanol stress responses, Plos One, 8(7), 2013.
10.1371/journal.pone.0068886
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  07/10/2013 Ma, Q., Liu, B., Zhou, C., Yin , Y., Li, G., Xu, Y., An integrated toolkit for accurate prediction and analysis of cis regulatory motifs at a genome scale, Bioinformatics, 29 (18) pp 2261-2268, 2013.
10.1093/bioinformatics/btt397
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  07/08/2013 GhattyVankataKrishna, P., Uberbacher, E.C., Cheng, X., Effect of the amyloid B hairpin's structure on the handedness of helices formed by its aggregates, FEBS Letters, 587(16), 2649-2655, 2013.
10.1016/j.febslet.2013.06.050
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  07/08/2013 Meng, X., Foston, M., Leisen, J., DeMartini, J., Wyman, C.E., Ragauskas, A.J., Determination of porosity of lignocellulosic biomass before and after pretreatment by using Simons' stain and NMR techniques, Bioresource Technology, 144:467-76, 2013.
10.1016/j.biortech.2013.06.091
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  06/01/2013 Zeng, Y., Zhao, S., Yang, S., Ding, S., Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels, Current Opinion in Biotechnology, 27 (38-45), 2013.
10.1016/j.copbio.2013.09.008
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  05/01/2013 Wyman, C.E., Introduction, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 1, pp 1-20, 2013.
10.1002/9780470975831
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  05/01/2013 Trajano, H.L., Wyman, C.E., Fundamentals of Biomass Pretreatment at Low pH, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 6, pp 103-123, 2013.
10.1002/9780470975831.ch6
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  05/01/2013 Sannigrahi, P., Ragauskas, A.J., Fundamentals of biomass pretreatment by fractionation, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 10, pp 201-219, 2013.
10.1002/9780470975831.ch10
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  05/01/2013 Kumar, R., Wyman, C.E., Physical and chemical features of pretreated biomass that influence macro-micro-accessibility and biological processing, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 14, pp 281-296, 2013.
10.1002/9780470975831.ch14
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  05/01/2013 Pu, Y., Hallac, B., Ragauskas, A.J., Plant biomass characterization: application of solution- and solid-state NMR spectroscopy, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 18, pp 369-387, 2013.
10.1002/9780470975831.ch18
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  05/01/2013 Qing, Q., Li, H., Kumar, R., Wyman, C.E., Xylooligosaccharides production, quantification, and characterization in context of lignocellulosic biomass pretreatment, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 19, pp 391-410, 2013.
10.1002/9780470975831.ch19
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  05/01/2013 Demartini, J., Wyman, C.E., High-throughput pretreatment and hydrolysis systems for screening biomass species in aqueous pretreatment of plant biomass, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 22, pp 471-486, 2013.
10.1002/9780470975831.ch22
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  05/01/2013 Li, H., Gao, X., DeMartini, J.D., Kumar, R., Wyman, C.E., Application of high throughput pretreatment and co-hydrolysis system to thermochemical pretreatment. Part 2: dilute alkali, Biotechnology and Bioengineering, 2013
10.1002/bit.24951
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  05/01/2013 Ragauskas, A.J., When to accept no … to yesterday's solutions, Biofuels, Bioproducts and Biorefining, 7(3), 2013.
10.1002/bbb.1414
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  04/24/2013 Flowers, D., Thompson R.A., Bridwell, D., Wang, T., Trihn, C.T., SMET: Systematic multiple enzyme targeting - a method to rationally design optimal strains for target chemical overproduction, Biotechnology Journal, 8(5):605-18, .2013
10.1002/biot.201200233
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  04/17/2013 Ma, Q., Yin, Y., Schell, M.A., Zhang, H., Li, G., Xu, Y., Computational analyses of transcriptomic data reveal the dynamic organization of the Escherichia coli chromosome under different conditions, Nucleic Acids Research, 41(11):5594-603, 2013.
10.1093/nar/gkt261
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  04/11/2013 Elkins, J.G., Hamilton-Brehm, S.D., Lucas, S., Han, J., Lapidus, A., Cheng, J.F., Goodwin, L.A., Pitluck, S., Peters, L., Mikhailova, N., Davenport, K.W., Detter, J.C., Han, C.S., Tapia, R., Land, M.L., Hauser, L., Kyrpides, N.C., Ivanova, N.N., Pagani, I., Bruce, D., Woyke, T., Cottingham, R.W., Complete genome sequence of the hyperthermophilic sulfate-reducing bacterium thermodesulfobacterium geofontis OPF15, Genome Announcements, 1(2); e00162-13, 2013.
10.1128/genomeA.00162-13
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  04/04/2013 Passian, A., Tetard, L., Thundat, T., Comments on the paper 'A comprehensive modeling and vibration analysis of AFM microcantilevers subjected to nonlinear tip-sample interaction forces, Ultramicroscopy, 131:92-93, 2013.
10.1016/j.ultramic.2013.03.016
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