Biomass Deconstruction and Conversion

Research Overview | Research Publications | Intellectual Property

Optimizing Microbes for Biomass Deconstruction and Conversion. Kelsey Yee operates a process-controlled Applikon fermenter to evaluate how well Caldicellulosiruptor obsidiansis (a consolidated bioprocessing microbe) ferments simple sugars derived from poplar pretreated with dilute acid. [Image courtesy Oak Ridge National Laboratory]

Two key hypotheses drive Biomass Deconstruction and Conversion research at the BioEnergy Science Center (BESC):

  • Microorganisms can be engineered to enable consolidated bioprocessing (CBP)
  • Enzymes and microbial biocatalysts can be understood and engineered to synergize with recalcitrance-reducing plant modifications to achieve better biomass deconstruction.

To test these hypotheses, BESC targets three microbial CBP platforms: yeast, Clostridium thermocellum, and Caldicellulosiruptor species. In yeast, a robust industrial ethanol producer, researchers have pursued genome modifications aimed at improving the expression and activity of enzymes that will digest cellulose. Already natural cellulose degraders, the other two microbes are thermophilic anaerobes whose metabolism is being manipulated by BESC to produce useful products such as ethanol or butanol.

Studies of microbial cellulose utilization at all levels—including enzyme components, enzyme systems, pure cultures, and mixed cultures—demonstrate the increased effectiveness of microbial cultures compared with free enzymes acting in the absence of cells. Enabling CBP will reduce the need for pretreatment chemicals toxic to microbes and will reduce the energy required to deconstruct and convert biomass into biofuels.

Biomass Deconstruction and Conversion Research Results

Publications

  Publication Date Citation
DOI
  11/11/2014 Basen, M., Schut, G.J., Nguyen D.M.N., Lipscomb, G.L., Benn, R.A., Prybol, C.J., Vaccaro, B.J., Poole, F.L., Kelly, R.M., Adams, M.W.W., Single gene insertion drives bioalcohol production by a thermophilic archaeon, PNAS, 2014
10.1073/pnas.1413789111
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  11/10/2014 Archambault-Leger, V., Losordo, Z., Lynd, L.R., Energy, sugar dilution, and economic analysis of hot water flow-through pre-treatment for producing biofuel from sugarcane residues, Biofuels, Bioproducts and Biorefining, 2014
10.1002/bbb.1524
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  10/21/2014 Holwerda, E.K., Thorne, P.G., Olson, D.G., Amador-Noguez, D., Engle, N.L., Tschaplinski, T.J., van Dijken, J.P., Lynd, L.R., The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading, Biotechnology for Biofuels, 7:155 2014
10.1186/s13068-014-0155-1
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  10/16/2014 Vishnivetskaya, T.A., Hamilton-Brehm, S.D., Podar, M., Mosher, J.J., Palumbo, A.V., Phelps, T.J., Keller, M., Elins, J.G., Community analysis of plant biomass-degrading microorganisms from Obsidian Pool, Yellowstone National Park, Microbial Ecology, 2014
10.1007/s00248-014-0500-8
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  10/14/2014 Young, J., Chung, D., Bomble, Y.J., Himmel, M.E., Westpheling, J., Deletion of CelA, the Most Abundant Cellulase in Caldicellulosiruptor bescii, Reveals its Crucial Role in the Deconstruction of Lignocellulosic Biomass, Biotechnology for Biofuels, 2014
10.1186/s13068-014-0142-6
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  09/16/2014 Groom, J., Chung, D., Young, J., Westpheling, J., Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalis generates a new host for the analysis of biomass deconstruction, Biotechnology for Biofuels, 7(1):132, 2014.
10.1186/s13068-014-0132-8
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  08/01/2014 Archambault-Leger, V., Shao, X., Lynd, L.R., Simulated performance of reactor configurations for hot-water pretreatment of sugarcane bagasse, ChemSusChem, 2014
10.1002/cssc.201402087
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  07/22/2014 Xiao, L., Wei, H., Himmel, M.E., Jameel, H., Kelley, S.S., NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review, Frontiers in Plant Science, 5:388, 2014.
0.3389/fpls.2014.00388
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  06/06/2014 Currie, D.H., Guss, A.M., Herring, C.D., Giannone, R.J., Johnson, C.M., Lankford, P.K., Brown, S.D., Hettich, R.L., Lynd, L.R., Profile of secreted hydrolases, associated proteins, and SipA in thermoanaerobacterium saccharolyticum during the degradation of hemicellulose, Applied and Environmental Microbiology, 80(16) 5001-5011, 2014.
10.1128/AEM.00998-14
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  06/05/2014 Mearls, E.B., Lynd, L.R., The identification of four histidine kinases that influence sporulation in Clostridium thermocellum, Anaerobe, 1:11 (109-19) 2014
10.1016/j.anaerobe.2014.06.004
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  06/02/2014 Chung, D., Cha, M., Guss, A.M., Westpheling, J., Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii, PNAS, 2014
10.1073/pnas.1402210111
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  04/30/2014 Lin, P.P., Rabe, K.S., Takasumi, J.L., Kadisch, M., Arnold, F.H., Liao, J.C., Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius, Metabolic Engineering, 24:1-8, 2014.
10.1016/j.ymben.2014.03.006
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  04/13/2014 Myung, S., Rollin, J., You, C., Sun, F., Chandrayan, S., Adams, M.W.W., Zhang, Y.-H P., In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose , Metabolic Engineering, 24:70-77, 2014.
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  04/04/2014 Biswas, R., Persad, A., Bisaria, V.S., Production of Cellulolytic Enzymes, Bioprocessing of Renewable Resources to Commodity Bioproducts, Chapter 5, pp 105-132, 2014.
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  04/01/2014 Kostylev, M., Alahuhta, M., Chen, M., Brunecky, R., Himmel, M.E., Lunin, V.V., Brady, J., Wilson, D.B., Cel48A From Thermobifida fusca: Structure and Site Directed Mutagenesis of Key Residues, Biotechnology and Bioengineering, 114:4 (664-673) 2013
10.1002/bit.25139
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  02/07/2014 Biswas, R., Prabhu, S., Lynd, L.R., Guss, A. M., Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of clostridium thermocellum, Plos One, 9(2), 2014.
10.1371/journal.pone.0086389
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  01/29/2014 Chen, M., Kostylev, M., Bomble, Y.J., Crowley, M.F., Himmel, M.E., Wilson, D.B., Brady, J.W., Experimental and modeling studies of an unusual water-filled pore structure with possible mechanistic implications in family 48 cellulases, The Journal of Physical Chemistry B, 118:9 (2306-2315) 2014
0.1021/jp408767j
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  01/14/2014 Kostylev, M., Wilson D., A Distinct Model of Synergism between a Processive Endocellulase (TfCel9A) and an Exocellulase (TfCel48A) from Thermobifida fusca, Applied and Environmental Microbiology, 80:1 2014
10.1128/AEM.02706-13
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  01/12/2014 Archambault-Leger, V., Lynd, L.R., Fluid mechanics relevant to flow through pretreatment of cellulosic biomass, Bioresource Technology, 157; (278-283) 2014.
10.1016/j.biortech.2014.01.035
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  01/12/2014 Basen, M., Rhaesa, A.M., Kataeva, I., Prybol, C.J., Scott, I.M., Pools, F.L., Adams, M.W.W., Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophililic bacterium Caldicellulosiruptor bescii, Bioresource Technology, 152 (384-392) 2013.
10.1016/j.biortech.2013.11.024
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  01/01/2014 You, C., Zhang, Y.-H. P., Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR, DNA Cloning and Assembly Methods Methods in Molecular Biology, Volume 1116, 2014, pp 183-192.
10.1007/978-1-62703-764-8_13
  01/01/2014 Zurawski, J.W., Blumer-Schuette, S.E., Conway, J.M., Kelly, R.M., The Extremely Thermophilic Genus Caldicellulosiruptor: Physiological and Genomic Characteristics for Complex Carbohydrate Conversion to Molecular Hydrogen, Microbial BioEnergy: Hydrogen Production, chapter 8, pp 177-195, 2014.
ISBN 978-94-017-8553-2
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  01/01/2014 Brunecky, R., Donohoe, B.S., Selig, M.H., Wei, H., Resch, M., Himmel, M.E., Basic Biological Research Relevant to Feestock Conversion, Compendium of Bioenergy Plants: Corn, Chapter 2, pp 33-69, 2014.
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  12/20/2013 Brunecky, R., Alahuhta, M., Xu, Q., Donohoe, B.S., Crowley, M.F., Kataeva, I.A., Yang, S.J., Resch, M. G., Adams, M.W.W., Lunin, V.V., Himmel, M.E., Bomble, Y.J., Revealing nature's cellulase diversity: the digestion mechanism of caldicellulosiruptor bescii celA, Science, 342 (6165): 1513-1516, 2013.
10.1126/science.1244273
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  12/12/2013 Reed, P.T., Izquierdo, J.A., Lynd, L.R., Cellulose fermentation by C. thermocellum and a mixed consortium in an automated repetitive batch reactor, Bioresource Technology, 155 (50-56) 2013
10.1016/j.biortech.2013.12.051
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  11/30/2013 Alahuhta, M., Adney, W.S., Himmel, M.E., Lunin, V.V., Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 A resolution, Acta Crystallographica Section F Structural Biology and Crystallization Communications, F69, 1335-1338, 2013.
10.1107/S1744309113030005
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  11/29/2013 Dumitrache, A., Wolfaardt, G.M., Allen, D.G., Liss, S.N., Lynd, L.R., Tracking the cellulolytic activity of clostridium thermocellum biofilms, Biotechnology for Biofuels, 6:175 2013
10.1186/1754-6834-6-175
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  11/18/2013 Ding, S.Y., Overview of Lignocellulose: Structure and Chemistry, Biological Concerstion of Biomass for Fuels and Chemicals: Exploration from Natural Utilization Systems, 10:2 (14-25) 2013
10.1039/9781849734738-00014
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  11/15/2013 Dykstra, A., St. Brice, L., Rodriguez Jr., M., Raman, B., Izquierdo, J., Cook, K.D., Lynd, L.R., Hettich, R.L., Development of a multi-point quantitation method to simultaneously measure enzymatic and structural components of the Clostridium thermocellum Cellulosome protein complex, Journal of Proteome Research, 13:2 (692-701) 2013
10.1021/pr400788e
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  11/09/2013 Bhandiwad, A., Shaw, A.J., Guss, A., Guseva, A., Bahl, H., Lynd, L.R., Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production, Metabolic Engineering, 21:71-25, 2014.
10.1016/j.ymben.2013.10.012
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  10/01/2013 Cha, M., Wang, H., Chung, D., Bennetzen, J.L., Westpheling, J., Isolation and bioinformatic analysis of a novel transposable element, ISCbe4, from the hyperthermophilic bacterium, caldicellulosiruptor bescii, Journal of Industrial Microbiology and Biotechnology, 40, (12) p. 1443-8, 2013.
10.1007/s10295-013-1345-8
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  09/27/2013 Sathitsuksanoh, N., Xu, B., Zhao, B., Zhang, Y.-H. P., Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment, Plos One, 8:9 e73523 2013
10.1371/journal.pone.0073523
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  08/30/2013 Xi, Q., Ding,S.Y., Brunecky, R., Bomble, Y.J., Himmel, M.E., Baker, J.O., Improving activity of minicellulosomes by integration of intra- and intermolecular synergies, Biotechnology for Biofuels, 6:126, 2013.
10.1186/1754-6834-6-126
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  08/08/2013 Taghavi, S., Izquierdo, J.A., van der Lelie, D., Complete Genome Sequence of Clostridium sp. Strain DL-VIII, a Novel Solventogenic Clostridium Species Isolated from Anaerobic Sludge, Genome Announcements, 1 (4), 2013.
10.1128/genomeA.00605-13.
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  08/01/2013 Blumer-Schuette, S.E., Sander, K.E., Bayer, E.A., Zurawski, J.V., Conway, J.M., Adams, M.W.W., Kelly, R.M., Thermophilic lignocellulose deconstruction, FEMS Microbial Review, 38:3 (393-448) 2014
10.1111/1574-6976.12044
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  07/12/2013 Zhang, X.Z., Zhang, Y.H.P., Cellulases: Characteristics, sources, production, and applications, Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers, Chapter 8, pp 131- 146, 2014.
10.1002/9781118642047.ch8
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  07/09/2013 Kostylev, M., Wilson, D., Two-parameter kinetic model based on a time-dependent activity coefficient accurately describes enzymatic cellulose digestion, Biochemistry, 52 (33), pp 5656–5664, 2013.
10.1021/bi400358v
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  06/15/2013 Atalla, R.S., Crowley, M.F., Himmel, M.E., Atalla, R.H., Irreversible transformations of native celluloses, upon exposure to elevated temperatures, Carbohydrate Polymers, 100, 2-8, 2013.
10.1016/j.carbpol.2013.06.007,
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  06/01/2013 Brown, S.D., Klingeman, D.M., Johnson, C.M., Clum, A., Aerts, A., Salamov, A., Sharma, A., Zane, M., Barry, K., Grigoriev, I.V., Davison, B.D., Lynd, L.R., Gilna, P., Hau, H., Hogsett, D.A., Froehlich, A.C., Genome sequences of industrially relevant Saccharomyces cerevisiae Strain M3707, isolated from a sample of distillers yeast and four haploid derivatives, Genome Announcements, vol. 1 no. 3, 2013.
10.1128/genomeA.00323-13
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  06/01/2013 Chung, D., Farkas, J., Westpheling, J., Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement, Biotechnology for Biofuels, 6:82, 2013.
10.1186/1754-6834-6-82
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  06/01/2013 Cha, M., Chung, D., Elkins, J.G., Guss, A.M., Westpheling, J., Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass, Biotechnology for Biofuels, 6:85, 2013.
10.1186/1754-6834-6-85
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  05/01/2013 Lynd, L., Laser, M., Cellulosic Biofuels: Importance, Recalcitrance, and Pretreatment, Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals, (ed Wyman, C.E.), chapter 2, pp 17-21, 2013.
10.1002/9780470975831.ch2
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  04/15/2013 van der Veen, D., Lo, J., Brown, S.D., Johnson, C.M., Tschaplinski, T.J., Martin,k M., Engle, N.L., van den Berg, R.A., Argyros, A.D., Caiazza, N.C., Guss, A.M., Lynd, L.R., Characterization of Clostridium thermocellum strains with disrupted fermentation end product pathways, 40(7), 725-734, 2013.
10.1007/s10295-013-1275-5
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  04/15/2013 Wang, Z.W., Lee, S.H., Elkins, J.G., Li, Y., Hamilton-Brehm, S., Morrell-Falvey, J.L., Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy, Journal of Environmental Sciences, 25(5), 849-856, 2013.
10.1016/S1001-0742(12)60104-1
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  04/11/2013 Holwerda, E.K., Lynd, L.R., Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum, Biotechnology and Bioengineering, 110(9):2389-94, 2013.
10.1002/bit.24914
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  04/11/2013 Holwerda, E.K., Ellis, L.D., Lynd, L.R., Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms, Biotechnology and Bioengineering, 110(9):2380-8, 2013.
10.1002/bit.24915
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  04/08/2013 Resch, M.G., Donohoe, B.S., Baker, J.O., Decker, S.R., Bayer, E.A., Beckham, G.T., Himmel, M.E., Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction, Energy and Environmental Science, 6; 1858-1867, 2013.
10.1039/C3EE00019B
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  04/03/2013 Chung, D., Cha, M., Farkas, J., Westpheling, J., Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus, PLoS ONE, 8(5): e62881, 2013.
10.1371/journal.pone.0062881
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  04/01/2013 You, C., Chen, H., Myung, S., Sathitsuksanoh, N., Ma. H., Zhang, X.Z., Li, J., Zhang, Y.-H.P., Enzymatic transformation of nonfood biomass to starch, PNAS, 110 no. 18 7182-7187 2013.
10.1073/pnas.1302420110
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  03/19/2013 Martin del Camp, J.S., Rollin, J., Myung, S., Chun, Y., Chandrayan, S., Patino, R., Adams, M.W.W., Zhang, Y.H., High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system, Angewandte Chemie, 52, 1-5, 2013.
10.1002/anie.201300766
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  03/10/2013 Chung, D., Farkas, J., Westpheling, Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus, Journal of Industrial Microbiology and Biotechnology, 40:517-521, 2013.
10.1007/s10295-013-1244-z
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  03/01/2013 Bhandiwad, A., Guseva, A., Lynd, L. R., Metabolic engineering of thermoanaerobacterium thermosaccharolyticum for increased n-butanol production, 3, 46-51, 2013.
10.4236/aim.2013.31007
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  03/01/2013 Alahuhta, M., Brunecky, R., Chandrayan, P., Kataeva, I., Adams, M.W.W., Himmel, M.E., Lunin, V.V., The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction, Acta Crystallographica Section D, D69, 534-539, 2013.
10.1107/S0907444912050512
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  02/22/2013 Zhou, J., Olson, D.G., Argyros, D.A., Deng, Y., van Gulik, W.M., van Dijken, J.P., Lynd, L.R., An atypical glycolysis in Clostridium thermocellum, Applied and Environmental Microbiology, 79(9):3000-8, 2013.
10.1128/​AEM.04037-12
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  02/01/2013 Currie, D.H., Herring, C.D., Guss, A.M., Olson, D.G., Hogsett, D.A., Lynd, L.R., Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolytim, Biotechnology for Biofuels, 6:32, 2013.
10.1186/1754-6834-6-32
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  02/01/2013 Fried, D.B., Morais, S., Xu, Q., Ding, S.Y., Baker, J.O., Bomble, Y.J., Himmel, M.E., Bayer, E.A., Self-assembly and application of cellulosomal components, Bionanotechnology: Biological Self-assembly and its Applications, Chapter 2, 2013.
ISBN: 978-1-908230-16-4
  01/04/2013 Hamilton-Brehm, S.D., Gibson, R.A., Green, S.J., Hopmans, E.C., Schouten, S., van der Meer, M.T.J., Shields, J.P., Damste, J.S.S., Elkins, J.G., Thermodesulfobacterium geofontis sp. Nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park, Extremophiles, 17(2):251-63, 2013.
10.1007/s00792-013-0512-1)
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  12/10/2012 Shao, X., Lynd, L., Kinetic modeling of xylan hydrolysis in co- and countercurrent liquid hot water flow-through pretreatments, Bioresource Technology, 130:117-124, 2012.
10.1016/j.biortech.2012.11.109
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  12/01/2012 Olson, D.G., Giannone, R.J., Hettich, R.L., Lynd, L.R., The role of the CipA scaffoldin protein in cellulose solubilization as determined by targeted gene deletion and complementation in Clostridium thermocellum, Journal of Bacteriology, 195(4):733-9, 2012.
10.1128/​JB.02014-12
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  12/01/2012 Deng, Y., Olson, D.G., Zhou, J., Herring, C.D., Shaw, A., Lynd, L.R., Redirecting carbon Flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum, Metabolic Engineering, 15:151-8, 2012.
10.1016/j.ymben.2012.11.006
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  12/01/2012 Li, L.L., Taghavi, S., Izquierdo, J.A., van der Lelie, D., Complete genome sequence of Clostridium sp. Strain BNL1100, a cellulolytic mesophile isolated from corn stover, Journal of Bacteriology, 194(24), p 69820-6983, 2012.
10.1128/JB.01908-12
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  11/30/2012 Sathitsuksanoh, N., George, A., Zhang, Y-H P., New lignocellulose pretreatments using cellulose solvents: a review, Journal of Chemical Technology and Biotechnology, 88 (2), 169-180, 2012.
10.1002/jctb.3959
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  11/23/2012 Ding, S.Y., Liu, Y.S., Zeng, Y., Himmel, M.E., Baker, J.O., Bayer, E.A., How Does Plant Cell Wall Nanoscale Architecture Correlate With Enzymatic Digestibility?, Science, 338 (6110), 1055-1060, 2012.
10.1126/science.1227491
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  10/10/2012 Waller, B.H., Olson, D.G., Currie, D.H., Guss, A.M., Lynd, L.R., Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome as revealed by SNAP-tags , FEMS Microbiology Letters, 338(1):46-53, 2012.
10.1111/1574-6968.12029
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  09/28/2012 Podkaminer, K.K., Guss, A.M., Trajano, H.L., Hogsett, D.A., Lynd, L.R., Characterization of xylan utilization and discovery of a new endoxylanase in Thermoanaerobacterium saccharolyticum through targeted gene deletions, Applied and Environmental Microbiology, 78:8441-7, 2012
10.1128/AEM.02130-12
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  09/19/2012 Farkas, J., Chung, D., Cha, M., Copeland, J., Grayeski, P., Westpheling, J., Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii, Journal of Industrial Microbiology and Biotechnology, 40(1):41-9., 2012.
10.1007/s10295-012-1202-1
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  08/22/2012 Chung, D., Farkas, J., Huddleston, J., Olivar, E., Westpheling, J., Methylation by a unique α-class N4-Cytosine Methyltransferase is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725, PLoS One, 7(8): e43844, 2012.
10.1371/journal.pone.0043844
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  08/16/2012 Mearls, E.B., Izquierdo, J.A., Lynd, L.E., Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms, BMC Microbiology, 12:180, 2012.
10.1186/1471-2180-12-180
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  08/01/2012 Himmel, M.E., Decker, S.R., Johnson, D.K., Challenges for Assessing the Performance of Biomass Degrading Biocatalysts, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:1-8, 2012.
10.1007/978-1-61779-956-3_1
  08/01/2012 Ding, S.Y., Liu, Y.S., Imaging Cellulose Using Atomic Force Microscopy, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:23-30, 2012.
10.1007/978-1-61779-956-3_3
  08/01/2012 Zeng, Y., Himmel, M.E., Ding, S.Y., Coherent Raman Microscopy Analysis of Plant Cell Walls, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:49-60, 2012.
10.1007/978-1-61779-956-3_5
  08/01/2012 Pattathil, S., Avci, U., Miller, J.S., Hahn, M.G., Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:61-72, 2012.
10.1007/978-1-61779-956-3_6
  08/01/2012 Wilson, D.B., Kostylev, M., Cellulase Processivity, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908;93-99, 2012.
10.1007/978-1-61779-956-3_9
  08/01/2012 Liu, Y.S., Ding, S.Y., Himmel, M.E., Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908;129-140, 2012.
10.1007/978-1-61779-956-3_13
  08/01/2012 Gilbert, J., Li, Luen-Luen, Taghavi, S., McCorkle, S.M., Tringe, S., van der Lelie, D., Bioprospecting Metagenomics for New Glycoside Hydrolases, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908;141-151, 2012.
10.1007/978-1-61779-956-3_14
  08/01/2012 Hamilton-Brehm, S.D., Vishnivetskaya, T.A., Allman, S.L., Mielenz, J.R., Elkins, J.G., Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908;153-168, 2012.
10.1007/978-1-61779-956-3_15
  08/01/2012 Brunecky, R., Baker, J.O., Wei, H., Taylor, L.E., Himmel, M.E., Decker, S.R., Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. celluloyticus EI, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:197-211, 2012.
10.1007/978-1-61779-956-3_18
  08/01/2012 Corea, O.R.A., Ki, C., Cardenas, C.L., Davin, L.B., Lewis, N.G., Laser Microdissection and Genetic Manipulation Technologies to Probe Lignin Heterogeneity and Configuration in Plant Cell Walls, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:229-250, 2012.
10.1007/978-1-61779-956-3_20
  08/01/2012 Chen, Y.R., Sarkanen, S., Wang, Y.Y., Lignin-Degrading Enzyme Activities, Methods in Molecular Biology Biomass Conversion: Methods and Protocols, 908:251-268, 2012.
10.1007/978-1-61779-956-3_21
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  01/01/2011 Blumer-Schuette, S.E., Ozdemir, I., Mistry, D., Lucas, S., Lapidus, A., Cheng, J.F., Goodwin, L.A., Pitluck, S., Land, M.L., Hauser, L.J., Woyke, T., Mikhailova, N., Pati, A., Kyrpides, D., Ivanova, N., Detter, J.C., Walston-Davenport, K., Han, S., Adams, M.W.W., Kelly, R.M., Complete Genome Sequences for the Anaerobic, Extremely Thermophilic Plant Biomass-Degrading Bacteria Caldicellulosiruptor hydrothermalis, Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor kronotskyensis, Caldicellulosiruptor owensensis, and Caldicellulosiruptor lactoaceticus, Journal of Bacteriology, 193:6;1483-1484, 2011.
10.1128/​JB.01515-10
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  12/29/2010 Podkaminer, Kara K., Hogsett, David A., Shao, Xiongjun, Lynd, Lee R., Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at 50°C with Thermoanaerobacterium saccharolyticum ALK2, Biofuels and Environmental Biotechnology, 108(6), 2011.
10.1002/bit.23050
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  12/21/2010 Atsumi, S., Wu, T. Y., Machado, I. M. P., Huang, W. C., Chen, P. Y., Pellegrini, M., Liao, J. C., 2010, Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli, Molecular Systems, Volume 6, pp 11.
10.1038/msb.2010.98
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  12/01/2010 Huang, W. D., Zhang, Y. H. P., Analysis of biofuels production from sugar based on three criteria: Thermodynamics, bioenergetics, and product separation, Energy and Environmental Science, Vol 4, Issue 3, pp 784, 2011.
10.1039/c0ee0069h
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  12/01/2010 Beckham, Gregg T., Bomble, Yannick J., Bayer, Edward A., Himmel, Michael E., 2011, Applications of computational science for understanding enzymatic deconstruction of cellulose, Current Opinion in Biotechnology, Volume 22, pp 1-8.
10.1016/j.copbio.2010.11.005
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  11/12/2010 Sathitsuksanoh, Noppadon, Zhu, Zhiguang, Wi, Sungsool, Zhang, Y.H-P, Cellulose Solvent-Based Biomass Pretreatment Breaks Highly Ordered Hydrogen Bonds in Cellulose Fibers of Switchgrass, Biotechnology and Bioengineering, 2011;108: 521-529, 2010.
10.1002/bit.22964
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  10/22/2010 Blumer-Schuette, S.E., Lewis, D.L., Kelly, R.M., Phylogenetic, microbiological and glycoside hydrolase diversity within the extremely thermophilic, plant biomass-degrading genus Caldicellulosiruptor, Applied Environmental Microbiology, 2010 Dec;76(24):8084-92
10.1128/AEM.01400-10
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  10/10/2010 Zhang, Jiayi, Lynd, L. R., Ethanol Production from Paper Sludge by Simultaneous Saccharification and Co-Fermentation Using Recombinant Xylose-Fermenting Microorganisms, Biotechnology and Bioengineering, Vol. 107, No. 2., pp 235-244, October 2010.
10.1002/bit.22811
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  10/01/2010 Elkins, J.G., Lochner, A., Hamilton-Brehm, S.D., Davenport, K.W., Podar, M., Brown, S.D., Land, M.L., Hauser, L.J., Klingeman, D.M., Raman, B., Goodwin, L.A., Tapia, R., Meincke, L.J., Detter, J.C., Bruce, D.C., Han, C.S., Palumbo, A.V., Cottingham, R.W., Keller, M., Graham, D.E., Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47, Journal of Bacteriology, 192:22, pp 6099-6100, 2010.
10.1128/JB.00950-10
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  10/01/2010 Bergenstrahle, M.,Wohlert, J., Himmel, M. E., Brady, J. W., Simulation studies of the insolubility of cellulose, Carbohydrate Research, Volume 345, Issue 14, pages 2060-2066, 2010.
10.1016/j.carres.2010.06.017
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  10/01/2010 Wang, Z.W., Hamilton-Brehm, S.D., Lochner, A., Elkins, J.G., Morrell-Falvey, J.L., Mathematical modeling of hydrolysate diffusion and utilization in cellulolytic biofilms of the extreme thermophile Caldicellulosiruptor obsidiansis, Bioresource Technology, 102:3155-62, 2010.
10.1016/j.biortech.2010.10.104
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  08/30/2010 Rollin, J. A., Zhu, Z. G., Sathitsuksanoh, N., Zhang, Y. H. P., Increasing Cellulose Accessibility Is More Important Than Removing Lignin: A Comparison of Cellulose Solvent-Based Lignocellulose Fractionation and Soaking in Aqueous Ammonia, Biotechnology and Bioengineering, Volume 108, Issue 1, pp 22-30, 2011
10.1002/bit.22919
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  08/16/2010 Olson, Daniel G., Tripathi, Shital A., Giannone, Richard J., Lo, Jonathan, Caiazza, Nicky C., Hogsett, David A.,Hettich, Robert, Guss, Adam M., Dubrovsky, Genia, Lynd, Lee R., Deletion of Cel48S from Clostridium thermocellum, PNAS, Volume 107, Issue 41, pp 17727-17732, 2010.
10.1073/pnas.1003584107
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  08/02/2010 Zhang, X. Z., Zhang, Y. H. P., One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges, Engineering in Life Science, Volume 10, Issue 5, pp 398-406, 2010.
10.1002/elsc.201000011
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  08/01/2010 Tripathi, S.A., Olson, D.G., Argyros, D.A., Miller, B.B., Barrett, T.F., Murphy, D.M., McCool, JD., Warner, A.K., Rajgarhia, V.B., Lynd, L.R., Hogsett, D.A., Caiazza, N.C., Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant, Applied and Environmental Microbiology, Volume 76, Issue 19, pp 6591-6599, 2010.
10.1128/AEM.01484-10
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  07/22/2010 Moinuddin, Syed G.A., Jourdes, M., Laskas, D.D., Ki, C., Cardenas, C.L., Kim, K.W., Zhang, D., Davin, L. B., Lewis, N.G., Insights into lignin primary structure and deconstruction from Arabidopsis thaliana COMT (caffeic acid O-methyl transferase) mutant Atomt1 , Organic and Biomolecular Chemistry, 8(17):3928-3946, 2010.
10.1039/c004817h
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  07/19/2010 Bomble, Yannick J., Crowley, Michael F., Xu, Q.I., Himmel, Michael E., Computational Modeling in Lignocellulosic Biofuel Production, Modeling the Cellulosome Using Multiscale Models, chapter 4, pp 75-98
10.1021/bk-2010-1052.ch004
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  07/02/2010 Vuong, T.V., Wilson, D.B., Glycoside Hydrolases: Catalytic Base/Nucleophile Diversity , Biotechnology and Bioengineering, Volume 107, Issue 2, pages 195-205, 2010.
10.1002/bit.22838
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  07/01/2010 Voelker, SL; Lachenbruch, B; Meinzer, FC; Jourdes, M; Ki, CY; Patten, AM; Davin, LB; Lewis, NG; Tuskan, GA; Gunter, L; Decker, SR; Selig, MJ; Sykes, R; Himmel, ME; Kitin, P; Shevchenko, O; Strauss, S.H., Antisense Down‐Regulation of 4CL Expression Alters Lignification, Tree Growth, and Saccharification Potential of Field‐Grown Populas, Plant Physiology, 154 (2): 874‐ 886, 2010.
10.1104/pp.110.159269
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  07/01/2010 Muddiman, David, Andrews, Genna, Lewis, Derrick, Notey, Jaspreet, Kelly, Robert, Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor scccharolyticus by GeLC-MS2, Annuals of Bioanalytical Chemistry, 398:377–389, 2010.
10.1007/s00216-010-3955-6
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  07/01/2010 Muddiman, David, Andrews, Genna, Lewis, Derrick, Notey, Jaspreet, Kelly, Robert, Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting, Annuals of Bioanalytical Chemistry, 398:1, 391-404, 2010.
10.1007/s00216-010-3929-8
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  07/01/2010 Zhang, Y.H.P., Renewable carbohydrates are a potential high-density hydrogen carrier, International Journal of Hydrogen Energy, Volume 35, Issue 19, pp 10334-10342, 2010.
doi:10.1016/j.ijhydene.2010.07.132
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  07/01/2010 Alahuhta, Markus, Xu Qi, Brunecky, Roman, Adney, William S., Ding, Shi You, Himmel Michael E, Lunin, Vladimir V., Structure of a fibronectin type III-like module from Chostridium thermocellum, Structural Biology and Crystallization Communications, Volume 66, Issue Pt 8, Pages 878-80, 2010.
10.1107/S1744309110022529
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  07/01/2010 Alahuhta, M., Xu, Q., Bomble, Y.J., Brunecky, R., Adney, W.S., Ding, S-Y., Himmel, M.E, Lunin, V.V., The Unique Binding Mode of Cellulosomal CBM4 from Clostridium thermocellum Cellobiohydrolase A, Journal of Microbiology,Volume 402, Issue 2, pp 374-387, 2010.
10.1016/j.jmb.2010.07.028
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  06/01/2010 Zhang, Y. H. P., Sun, J. B., Zhong, J. J., Biofuel production by in vitro synthetic enzymatic pathway biotransformation, Current Opinion in Biotechnology, Volume 21, Issue 5, pp 663-669, 2010.
10.1016/j.copbio.2010.05.005
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  06/01/2010 Zhang, X.Z., Sathisuksanoh, N., Zhang, Y.H.P., Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, purfication, and synergy with family 48 cellobiohydrolase, Bioresource Technology, Volume 101, Issue 14 pp 5534-5538, 2010
10.1016/j.biortech.2010.01.152
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  06/01/2010 Elkins, J. G., Raman, B., Keller, M., Engineered microbial systems for enhanced conversion of lignocellulosic biomass, Current Opinion in Biotechnology, Volume 21, Issue 5, pp 657-662, 2010.
10.1016/j.copbio.2010.05.008
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  06/01/2010 Liu, W.J., Zhang, X.A., Zhang, Z.M., Zhang, Y.H.P., Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability, Applied and Environmental Microbiology, Volume 76, Issue 14, pp 4914-4917, 2010.
10.1128/AEM.00958-10
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  06/01/2010 Izquierdo, J. A., Sizova, M. V., Lynd, L. R., Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost, Applied and Environmental Microbiology, Volume 76, Issue 11, pp 3545-3553, 2010.
10.1128/AEM.02689-09
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  05/01/2010 Shaw, A., Joe, Hogsett, David A., Lynd, Lee R., Natural Competence in Thermoanaerobacter and Thermoanaerobacterium Species, Applied and Environmental Microbiology, Volume 76, Issue 14, pp 4713-4719, July 2010.
10.1128/AEM.00402-10
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  04/07/2010 Yang, S.H., Pelletier, D.A., Lu, T.Y.S, Brown, S.D., The Zymomonas mobilis regulator Hfq and related Saccharomyces cerevisiae proteins contribute to tolerance against multiple lignocellulosic pretreatment inhibitors, BMC Microbiology, 10;135, 2010.
10.1186/1471-2180-10-135
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  04/01/2010 Yang, S.H., Land, M.L., Klingeman, D.M., Pelletier, D.A., Lu, T.Y.S., Martin, S.L., Guo, H.B., Smith, J.C., Brown, S.D., Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae, PNAS, 107;23, pp 10398-10400, 2012.
10.1073/pnas.0914506107
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  04/01/2010 Myung, S., Wang, Y. R., Zhang, Y. H. P., Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: Characterization, metabolite stability, and its implications, Process Biochemistry, Volume 45, Issue 12, pp 1882-1887, 2010.
10.1016/j.procbio.2010.03.017
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  04/01/2010 Li, Y., Irvin, D.C., Wilson, D.B., Increased Crystalline Cellulose Activity via Combinations of Amino Acid Changes in the Family 9 Catalytic Domain and Family 3c Cellulose Binding Module of Thermobifida fusca Cel9A, Applied and Environmental Microbiology, Volume 76, Issue 8, pp 2582-2588, 2010.
10.1128/AEM.02735-09
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  04/01/2010 Selig, Michael J., Tucker, Melvin P., Sykes, R., Reichel, D., Brunecky, R., Himmel, M.E., Davis, M.F., Decker, S.R., Lignocellulose recalcitrance screening by integrated high- throughput hydrothermal pretreatment and enzymatic, Industrial Biotechnology , 6(2), 2010.
10.1089/ind.2010.0009
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  03/01/2010 Chen, Y., Sarkanen, S., Macromolecular replication during lignin biosynthesis, Phytochemistry, Volume 71, Issue 4, March 2010, Pages 453-462
10.1016/j.phytochem.2009.11.012
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  03/01/2010 Mielenz, J.R., Hogsett, D.A., Improving microbial robutsness using systems biology, Manual of Industrial Microbiology and Biotechnology, 3rd Edition, p. 605-620, 2010.
ISBN 978-1-55581-512-7
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  03/01/2010 Abulencia, C. B., Wells, S. M., Gray, K. A., Keller, M., Kreps, J.A., Accessing microbial communities relevant to biofuels production. in: R. H.Baltz, J. E.Davies, A. L.Demain (Eds.), Industrial Microbiology and Biotechnology, 3rd Edn, ASM press, Washington DC 2010, pp. 565–576.
ISBN 155581512X
  03/01/2010 Himmel, Michael E., Xu, Qi, Luo, Yonghua, Ding, Shi-You, Lamed, Raphael, Bayer, Edward A., Microbial enzyme systems for biomass conversion: emerging paradigms, Biofuels, March 2010, Vol. 1, No. 2, Pages 323-341, 2010.
10.1021/cm903135
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  03/01/2010 Ye, X., Rollin, J., Zhang, Y.H.P., Thermophilic α-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability, Journal of Molecular Catalysis B: Enzymatic, Volume 65, Issue 1-4, pp 110-116
10.1016/j.molcatb.2010.1001.1015
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  03/01/2010 Zhang, Z.Z., Zhang, Z., Zhu, Z., Sathitsuksanoh, N., Yang, Y., Zhang, Y.H.P., The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity, Applied Microbiology Biotechnology, 86(2):525-533, 2010.
http://dx.doi.org/10.1007/s00253-009-2231-1
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  12/18/2009 Hamilton-Brehm, S.D., Mosher, J.J., Vishnivetskaya, T., Podar, M., Carroll, S., Allman, S., Phelps, T.J., Keller, M., Elkins, J.G., Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Applied and Environmental Microbiology, 76:1014-1020. 2010.
10.1128/AEM.01903-09
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  12/15/2009 Vanfossen Amy L, Verhaart Marcel R A, Kengen Serve M W, Kelly Robert M., Carbohydrate utilization patterns for the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus reveal broad growth substrate preferences, Applied and Environmental Microbiology 75(24):7718-24, 2009.
10.1128/AEM.01959-09
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  12/11/2009 Bochner, Barry, Gomez, Vanessa, Ziman, Michael, Yang, Shihui, Brown, Steven D., Phenotype MicroArray Profiling of Zymomonas mobilis AM4, Applied Biochemistry and Biotechnology, May 2010; pp 116-123
10.1007/s12010-009-8842-2
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  12/01/2009 Jeremy C. Smith, Mark Nimlos, Mike Himmel, Mike Crowley, John Brady, Supercomputers Tackle BIOFUEL Production Problems, SciDAC, 2009.
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  12/01/2009 Sung-Jae Yang, Irina Kataeva, Juergen Wiegel, Yanbin Yin, Phuongan Dam, Ying Xu, Janet Westpheling and Michael W. W. Adams, Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. Nov., International Journal of Systematic and Evolutionary, Microbiology, Volume 60, Part 9, pp 2011-2015
10.1099/ijs.0.017731-0
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  12/01/2009 Decker, Stephen R., Brunecky, Roman, Tucker, Melvin, P., Himmel, Michael E., Selig, Michael J., High-throughput screening techniques for biomass conversion, Bioenergy Research, Volume 2, Number 4 / December, 2009.
10.1007/s12155-009-9051-0
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  12/01/2009 Mielenz, J.R., Bardsley, J.S., Wyman, C.E., Fermentation of soybean hulls to ethanol while preserving protein value, Bioresource Technology, Jul;100(14):3532-9, 2009.
10.1016/j.biortech.2009.02.044
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  11/01/2009 Zhang YHP. Production of Biocommodities and Bioelectricity by Cell-Free Synthetic Enzymatic Pathway Biotransformations: Challenges and Opportunities. Biotechnology and Bioengineering 2010;105(4):663-677.
10.1002/bit.22630
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  10/22/2009 Patten A.M., Jourdes, M., Cardenas, C.L., Laskas, D.D., Nakazawa, Y., Chung, B.Y., Franceschi, V.R., Davin, L.B., Lewis, N.G., Probing native lignin macromolecular configuration in Arabidopsis thaliana in specific cell wall types: Further insights into limited substrate degeneracy and assembly of the lignins of ref8, fah 1–2 and C4H::F5H lines, Molecular BioSystems, 6:3:pp 425-604, 2010.
10.1039/b819206e
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  10/01/2009 Podar, Mircea, Keller, Martin, Hugenholtz, Philip, 2009, Single Cell Whole Genome Amplification of Uncultivated Organisms, Microbial Monogr, Series; Volume 10/2009.
10.1007/7171_2008_10
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  10/01/2009 Yang, S.H., Pappas, K.M., Hauser, L.J., Land, M.L., Chen, G.L., Hurst, G.B., Pan, C.L., Kouvelis, V.N., Typas, M.A., Pelletier, D.A., Klingeman, D.M., Chang, Y.J., Samatova, N.F., Brown, S.D., Improved genome annotation for Zymomonas mobilis, Nature Biotechnology, 27;10, pp 893 - 894, 2009.
10.1038/nbt1009-893
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  09/25/2009 Sathitsuksanoh N, Zhu ZG, Ho T-J, Bai M-D, Zhang Y-HP,Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings Volume 101, Issue 13, July 2010, Pages 4926-4929, 2009.
10.1016/j.biortech.2009.09.081
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  09/01/2009 Y.H. Percival Zhang, Jiong Hong, and Xinhao Ye, Cellulase Assays, Biofuels: Methods and Protocols, Methods in Molecular Biology, Jonathan R. Mielenz (ed.), vol. 581; 2009 Chapter 14
10.1007/978-1-60761-214-8_14
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  09/01/2009 Liu W, Bevan DR, Zhang Y-HP,The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase, Applied Biochemistry and Biotechnology, 161:1-8, 2009.
10.1007/s12010-009-8782-x
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  07/01/2009 Zhu, Z. G., Sathitsuksanoh, N., Vinzant, T., Schell, D. J., McMillan, J. D., Zhang, Y. H. P., Comparative Study of Corn Stover Pretreated by Dilute Acid and Cellulose Solvent-Based Lignocellulose Fractionation: Enzymatic Hydrolysis, Supramolecular Structure, and Substrate Accessibility, Biotechnology and Bioengineering, (2009), Volume 103(4), 715-724
10.1039/b906065k
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  06/28/2009 Wang, Y. R., Zhang, Y. H. P., Cell-free protein synthesis energized by slowly-metabolized maltodextrin, BMC Biotechnology, 2009, 9:58.
10.1186/1472-6750-9-58
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  06/04/2009 Wang, Y., Zhang, Y.H.P., Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration, Microbial Cell Factories, 2009, 8:30.
10.1186/1475-2859-8-
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  06/01/2009 Kataeva IA, Yang SJ, Dam P, Poole FL, Yin Y, Zhou F, Chou WC, Xu Y, Goodwin L, Sims DR, Detter JC, Hauser LJ, Westpheling J, Adams WW, Genome Sequence of the Anaerobic, Thermophilic, and Cellulolytic Bacterium "Anaerocellum thermophilum" DSM 6725, Journal of Bacteriology, Jun;191(11):3760-1, 2009.
10.1128/JB.00256-09
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  05/28/2009 Sathitsuksanoh, N., Zhu, Z. G., Templeton, N., Rollin, J. A., Harvey, S. P., Zhang, Y. H. P., Saccharification of a Potential Bioenergy Crop, Phragmites australis (Common Reed), by Lignocellulose Fractionation Followed by Enzymatic Hydrolysis at Decreased Cellulase Loadings, Industrial and Engineering Chemistry Research, 48(13):6441-6447, 2009.
10.1021/ie900291s
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  05/01/2009 Yang, S.J., Kataeva, I., Hamilton-Brehm, S., Engle, N.D., Tschaplinski, T.J., Doeppke, Cr., Davis, M., Westpheling, J., Adams, M.W.W., Efficient degradation of lignocellulosic plant biomass without pretreatment by the thermophilic anaerobe, Anaerocellum thermophilium DSM 6725, Applied and Environmental Microbiology, 75(14):4762-4769, 2009
10.1128/AEM.00236-09
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  05/01/2009 Xu, J.C., Crowley, M.F., Smith, J.C., Building a Foundation for Structure-Based Cellulosome Design for Cellulosic Ethanol: Insight into Cohesin-Dockerin Complexation from Computer Simulation, Protein Science, 18;5, pp 949 - 959, 2009
10.1002/pro.105
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  04/22/2009 Raman, B., Pan, C., Hurst, G. B., Rodriguez, M., McKeown, C. K., Lankford, Samatova, N. F., Mielenz, J. R., Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis, Plos One, 4(4): e5271 (2009)
10.1371/journal.pone.0005271
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  04/01/2009 Liu, W.J., Hong, J., Bevan, D.R., Zhang, Y.H.P., Fast Identification of Thermostable Beta-Blucosidase Mutants on Cellobiose by a Novel Combinatorial Selection/Screening Approach, Biotechnology and Bioengineering, Volume 103 Issue 6, Pages 1087 - 1094, 2009.
10.1002/bit.22340
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  03/09/2009 Keller, Martin, Hettich, Robert, Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level, Microbiology and Molecular Biology Reviews, March 2009, p. 62-70, Vol. 73, No. 1
10.1128/MMBR.00028-08
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  02/01/2009 Li, L. L., McCorkle, S. R., Monchy, S., Taghavi, S., van der Lelie, D., Bioprospecting metagenomes: glycosyl hydrolases for converting biomass, Biotechnology for Biofuels, Volume 2, pp 11, 4:23, 2009.
10.1186/1754-6834-2-10
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  01/20/2009 Yang, S.H., Tschaplinski, T.J., Engle, N.L., Carroll, S.L., Martin, S.L., Davison, B.H., Palumbo, A.V., Rodriguez, M., Brown, S.D., Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations, BMC Genomics, 10;34; 2009.
10.1186/1471-2164-10-34
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  01/01/2009 Keller, M., Schadt, C.W., Palumbo, A.V., Environment to Organisms and Genomes and Backed. Karsten Zengler, (2009), ASM Press.
  01/01/2009 Ye, Xinhao, Yiran Wang, Robert C. Hopkins, Michael W. W. Adams, Barbara R. Evans, Jonathan R. Mielenz, Y.-H. Percival Zhang, Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails , ChemSusChem, (2009), 2(2), 149-52, 2009
10.1002/cssc.200900017
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  01/01/2009 Zhang Y-HP, Zhu Z, Rollin J, Sathisuksanoh N. 2010. Chapter 20. Advances in cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF). ACS Symposium Series 1033: 365-379 (Cellulose solvents: For analysis, shaping and chemical modification), Oxford University Press, UK, ISBN13: 9780841200067.
10.1021/bk-2010-1033.ch020.
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  01/01/2009 Zhang YHP. A sweet out-of-the-box solution to the hydrogen economy: is the sugar-powered car science fiction? Energy and Environmental Science 2009;2(3):272-282.
10.1039/ b818694d
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  01/01/2009 Zhiguang Zhu, Noppadon Sathitsuksanoha and Y.-H. Percival Zhang, Direct quantitative determination of adsorbed cellulase on lignocellulosic biomass with its application to study cellulase desorption for potential recycling, Analyst, 2009 1347:2267-2272
10.1039/b906065k
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  08/01/2008 Moxley, Geoffrey, Zhiguang Zhu, and Y.-H. Percival Zhang ,Efficient Sugar Release by the Cellulose Solvent-Based Lignocellulose Fractionation Technology and Enzymatic Cellulose Hydrolysis , Journal of Agricultural and Food Chemistry, 56 (17), 7885–7890, 2008
10.1021/jf801303f
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  08/01/2008 Yuan, Joshua S., Kelly H. Tiller, Hani Al-Ahmad, Nathan R. Stewart, C. Neal Stewart, Jr., Plants to power: bioenergy to fuel the future, Trends in Plant Science, Vol. 13(8), 421-429, 2008
10.1016/j.tplants.2008.06.001
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  07/01/2008 Hong, Jiong, Xinhao Ye, Yiran Wang and Y.-H. Percival Zhang Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent, Analytica Chimica Acta, 621:193-199, 2008.
10.1016/j.aca.2008.05.041
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  06/20/2008 Hong, Jiong, Yiran Wang, Xinhao Ye and Y.-H. Percival Zhang , Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage, Journal of Chromatography, A Volume 1194, Issue 2, 20, Pages 150-154, June 2008.
10.1016/j.chroma.2008.04.048
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  06/01/2008 Blumer-Schuette, Sara E., Kataeva, Irina, Westpheling, Janet, Adams, Michael W.W., Kelly, Robert M., 2008, Extremely thermophilic microorganisms for biomass conversion; status and prospects, Current Opinion in Biotechnology, 19(3), 210-217, 2008.
10.1016/j.copbio.2008.04.007
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  04/01/2008 Ljungdaho, L.G., Kataeva, I.A., Uversky, V.V., Contribution of domain interactions and calcium binding to the stability of carbohydrate-Active enzymes, Bioenergy, Judy D. Wall, Caroline S. Harwood and Arnold Demain eds., ASM Press, washington, DC, p.115-127, 2008.
ISBN-13: 9781555814786
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